García-Lara J, Picardeau M, Hinnebusch B J, Huang W M, Casjens S
Department of Microbiology, University of Georgia, Athens 30602, USA.
J Mol Microbiol Biotechnol. 2000 Oct;2(4):447-54.
The identification of chromosomal and episomal origins of replication in the genome of the causative agent of Lyme disease, the spirochete Borrelia burgdorferi, has been greatly facilitated by genomics. Analysis of genome features, including strand compositional asymmetries, organizational similarities to other bacterial origins of replication, and the presence of homologues of genes involved in replication and partitioning, have contributed to the identification of a collection of putative origins of replication within the Borrelia genome. This analysis has provided the basis for the experimental verification of origins in the linear chromosome and in the linear plasmid Ip28-2. Information generated during the study of these origins will significantly contribute to the understanding of the mechanisms of replication and partitioning in Borrelia.
基因组学极大地推动了莱姆病病原体——螺旋体伯氏疏螺旋体基因组中染色体和附加型复制起点的鉴定。对基因组特征的分析，包括链组成不对称性、与其他细菌复制起点的组织相似性，以及参与复制和分配的基因同源物的存在，有助于在伯氏疏螺旋体基因组中鉴定出一系列推定的复制起点。该分析为线性染色体和线性质粒Ip28-2中复制起点的实验验证提供了基础。在这些复制起点研究过程中产生的信息将显著有助于理解伯氏疏螺旋体的复制和分配机制。
