使用高效液相色谱法对马血浆和尿液中苯妥英及其代谢物进行定量分析。
Quantification of phenytoin and its metabolites in equine plasma and urine using high-performance liquid chromatography.
作者信息
Guan F, Uboh C E, Soma L R, Birks E K, Teleis D, Rudy J A, Watson A O, Tsang D S
机构信息
University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, Kennett Square 19348, USA.
出版信息
J Chromatogr B Biomed Sci Appl. 2000 Sep 15;746(2):209-18. doi: 10.1016/s0378-4347(00)00330-3.
A reliable and sensitive method for the extraction and quantification of phenytoin (5,5'-diphenylhydantoin), its major metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) and minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) in horse urine and plasma is described. The method involves the use of solid-phase extraction (SPE), liquid-liquid extraction (LLE), enzyme hydrolysis (EH) and high-performance liquid chromatography (HPLC). The minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) was not present in a reliably quantifiable concentration in all samples. The new method described was successfully applied in the pharmacokinetic studies and elimination profile of phenytoin and p-HPPH following oral or intravenous administration in the horse.
本文描述了一种可靠且灵敏的方法，用于提取和定量马尿液及血浆中的苯妥英（5,5'-二苯基乙内酰脲）、其主要代谢物5-(对羟基苯基)-5-苯基乙内酰脲（p-HPPH）以及次要代谢物5-(间羟基苯基)-5-苯基乙内酰脲（m-HPPH）。该方法包括使用固相萃取（SPE）、液液萃取（LLE）、酶水解（EH）和高效液相色谱（HPLC）。次要代谢物5-(间羟基苯基)-5-苯基乙内酰脲（m-HPPH）在所有样品中均未以可靠可定量的浓度存在。所描述的新方法成功应用于马口服或静脉给药后苯妥英和p-HPPH的药代动力学研究及消除情况分析。
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