Guan F, Uboh C E, Soma L R, Birks E K, Teleis D, Rudy J A, Watson A O, Tsang D S
University of Pennsylvania School of Veterinary Medicine Department of Clinical Studies, Kennett Square 19348, USA.
J Chromatogr B Biomed Sci Appl. 2000 Sep 15;746(2):209-18. doi: 10.1016/s0378-4347(00)00330-3.
A reliable and sensitive method for the extraction and quantification of phenytoin (5,5'-diphenylhydantoin), its major metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (p-HPPH) and minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) in horse urine and plasma is described. The method involves the use of solid-phase extraction (SPE), liquid-liquid extraction (LLE), enzyme hydrolysis (EH) and high-performance liquid chromatography (HPLC). The minor metabolite, 5-(m-hydroxyphenyl)-5-phenylhydantoin (m-HPPH) was not present in a reliably quantifiable concentration in all samples. The new method described was successfully applied in the pharmacokinetic studies and elimination profile of phenytoin and p-HPPH following oral or intravenous administration in the horse.
本文描述了一种可靠且灵敏的方法，用于提取和定量马尿液及血浆中的苯妥英（5,5'-二苯基乙内酰脲）、其主要代谢物5-(对羟基苯基)-5-苯基乙内酰脲（p-HPPH）以及次要代谢物5-(间羟基苯基)-5-苯基乙内酰脲（m-HPPH）。该方法包括使用固相萃取（SPE）、液液萃取（LLE）、酶水解（EH）和高效液相色谱（HPLC）。次要代谢物5-(间羟基苯基)-5-苯基乙内酰脲（m-HPPH）在所有样品中均未以可靠可定量的浓度存在。所描述的新方法成功应用于马口服或静脉给药后苯妥英和p-HPPH的药代动力学研究及消除情况分析。
