Del Gobbo V, Giganti M G, Zenobi R, Villani V, Premrov M G
Department of Experimental Medicine and Biochemical Sciences, School of Medicine, University of Rome Tor Vergata, Italy.
Am J Reprod Immunol. 2000 Oct;44(4):214-21. doi: 10.1111/j.8755-8920.2000.440405.x.
The present study examines the hypothesis that the elevated levels of transforming growth factor (TGF)-beta1 and interleukin (IL)-10 would be protective for the fetus survival during pregnancy-induced hypertension (PIH). Moreover, we evaluate the IL-12 and IL-15 serum concentrations and their relationships with PIH.
Serum samples were obtained before the onset of labor from control and PIH groups. Cytokine concentrations were determined by Enzyme-Linked Immunoadsorbent Assay.
Our data show that PIH women have significantly higher TGF-beta1 and IL-10 concentrations with respect to control groups (P = 0.0001). Similarly, macrophages from the PIH placentas produce in vitro more elevated TGF-beta1 and IL-10 levels compared to normal pregnant ones (P = 0.02), also in the absence of LPS stimulation. IL-12 and IL-15 serum concentrations were not detectable in all pregnant groups.
We have found that PIH women have elevated concentrations of anti-inflammatory/immunosuppressive cytokines, suggesting their important role in fetal allograft protection during the normal and pathological pregnancy.
本研究检验了如下假设，即转化生长因子（TGF）-β1和白细胞介素（IL）-10水平升高对妊娠期高血压疾病（PIH）期间胎儿存活具有保护作用。此外，我们评估了IL-12和IL-15的血清浓度及其与PIH的关系。
在分娩开始前从对照组和PIH组获取血清样本。通过酶联免疫吸附测定法测定细胞因子浓度。
我们的数据显示，与对照组相比，PIH女性的TGF-β1和IL-10浓度显著更高（P = 0.0001）。同样，与正常孕妇相比，PIH胎盘的巨噬细胞在体外产生的TGF-β1和IL-10水平更高（P = 0.02），且不存在脂多糖刺激。在所有孕妇组中均未检测到IL-12和IL-15的血清浓度。
我们发现PIH女性的抗炎/免疫抑制细胞因子浓度升高，表明它们在正常和病理妊娠期间对胎儿同种异体移植的保护中发挥重要作用。
