Kanangat S, Bronze M S, Meduri G U, Postlethwaite A, Stentz F, Tolley E, Schaberg D
Memphis Lung Research Program, Department of Medicine, Divisions of Pulmonary and Critical Care Medicine, University of Tennessee, Memphis, Tennessee, USA.
J Infect Dis. 2001 Jan 1;183(1):65-69. doi: 10.1086/317645. Epub 2000 Nov 10.
Replication of Staphylococcus aureus is significantly enhanced in the presence of recombinant interleukin (IL)-1beta. In this study, specific binding of IL-1beta to the surface of S. aureus significantly increased growth of S. aureus in the presence of IL-1beta and IL-1ra in a concentration-dependent manner. Although IL-1ra enhanced the growth of S. aureus, there was a significant reduction in IL-1beta-mediated growth enhancement of S. aureus when 25-fold excess amounts of IL-1ra (in comparison with the IL-1beta concentration) were present in the culture medium. Thus, IL-1beta may influence the growth of S. aureus through a receptor-mediated event. By using 5 linear peptides spanning limited regions of IL-1beta, the growth-promoting regions were localized to amino acid residues 118-147 and 208-240. These results build on the newly evolved concept of direct interactions between the soluble mediators of inflammation and infectious agents.
在重组白细胞介素（IL）-1β存在的情况下，金黄色葡萄球菌的复制显著增强。在本研究中，IL-1β与金黄色葡萄球菌表面的特异性结合在IL-1β和IL-1ra存在时以浓度依赖性方式显著增加了金黄色葡萄球菌的生长。虽然IL-1ra促进了金黄色葡萄球菌的生长，但当培养基中存在25倍过量的IL-1ra（与IL-1β浓度相比）时，IL-1β介导的金黄色葡萄球菌生长增强显著降低。因此，IL-1β可能通过受体介导的事件影响金黄色葡萄球菌的生长。通过使用跨越IL-1β有限区域的5种线性肽，将生长促进区域定位到氨基酸残基118 - 147和208 - 240。这些结果建立在炎症可溶性介质与感染因子之间直接相互作用这一新出现的概念之上。
