Zurita M, Vaquero J, Oya S
Neuroscience Research Unit of the Mapfre-Medicine Foundation, Puerta de Hierro Clinic, Autonomous University, Madrid, Spain.
Surg Neurol. 2000 Aug;54(2):117-25. doi: 10.1016/s0090-3019(00)00287-1.
To determine the influence of different nervous tissue grafts on the regenerative activity of chronically injured spinal cord, an experimental study examining the expression of the proliferating cell nuclear antigen (PCNA) in chronically injured spinal cord subjected to neural grafting was performed.
Three months after induced spinal cord injury, paraplegic Wistar rats were subjected to grafting of neural tissue. Grafts consisted of fetal brain cortex, fetal spinal cord, crushed adult peripheral nerve tissue, or fetal brain cortex combined with crushed adult peripheral nerve tissue. Four months later, the spinal cord was removed and the grafted zone was studied by means of immunohistochemical demonstration of PCNA.
Different patterns of PCNA expression were recorded in the different experimental groups. PCNA-immunostained cells in injured spinal cord tissue, mainly ependymal cells and astrocytes, increased when co-transplantation of fetal brain cortex and crushed adult peripheral nerve tissue was used, in comparison to other neural donor tissues. In the grafted tissue, proliferative activity was greater when fetal brain cortex, alone or with peripheral nerve, was used, in comparison to the use of fetal spinal cord or adult peripheral nerve tissue. Nevertheless, the number of PCNA-positive cells does not seem to be influenced by the presence of peripheral nerve tissue in the donor tissue.
Our present findings suggest the effectiveness of co-transplantation of peripheral nerve tissue and fetal brain tissue in attempts at spinal cord reconstruction after injury.
为了确定不同神经组织移植对慢性损伤脊髓再生活性的影响，进行了一项实验研究，检测接受神经移植的慢性损伤脊髓中增殖细胞核抗原（PCNA）的表达。
诱导脊髓损伤3个月后，对截瘫的Wistar大鼠进行神经组织移植。移植组织包括胎儿脑皮质、胎儿脊髓、粉碎的成年周围神经组织或胎儿脑皮质与粉碎的成年周围神经组织的组合。4个月后，取出脊髓，通过PCNA的免疫组织化学检测研究移植区域。
不同实验组记录到不同的PCNA表达模式。与其他神经供体组织相比，当使用胎儿脑皮质和粉碎的成年周围神经组织共同移植时，损伤脊髓组织中PCNA免疫染色的细胞（主要是室管膜细胞和星形胶质细胞）增加。在移植组织中，与使用胎儿脊髓或成年周围神经组织相比，单独使用胎儿脑皮质或与周围神经一起使用时增殖活性更高。然而，PCNA阳性细胞的数量似乎不受供体组织中周围神经组织存在的影响。
我们目前的研究结果表明，在损伤后脊髓重建的尝试中，周围神经组织与胎儿脑组织共同移植是有效的。
