Jankun M
Department of Evolutionary Ecology, University WM in Olsztyn, Olsztyn-Kortowo, Poland.
Cytobios. 2000;103(403):79-89.
Replication banding patterns have been obtained from in vivo treatment of Salmo trutta morpha trutta chromosomes using a modification of the 5-BrdU technique and in kidney cultures using the fluorochrome photolysis Giemsa (FPG) staining method. Each chromosome pair was identified in the karyotype based on the banding pattern, chromosome size, and centromere position. The standard karyotype of sea trout has been proposed. Similarities of replication, and restriction enzyme banding patterns of chromosomes 8 and 9, and of chromosomes 11 and 12, are discussed. Fluorescence in situ hybridization was used to determine the location of telomeric sequences.
利用5-溴脱氧尿苷（5-BrdU）技术的一种改良方法，对海鳟（Salmo trutta morpha trutta）染色体进行体内处理，并在肾培养物中使用荧光染料光解吉姆萨（FPG）染色法，获得了复制带型。根据带型、染色体大小和着丝粒位置，在核型中鉴定出每对染色体。提出了海鳟的标准核型。讨论了第8和9号染色体以及第11和12号染色体在复制和限制酶带型方面的相似性。利用荧光原位杂交技术确定端粒序列的位置。
