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大肠杆菌K12中谷胱甘肽生物合成基因gshA的定位。

Mapping of gshA, a gene for the biosynthesis of glutathione in Eschericha coli K12.

作者信息

Apontoweil P, Berends W

出版信息

Mol Gen Genet. 1975 Nov 24;141(2):91-5. doi: 10.1007/BF00267676.

DOI:10.1007/BF00267676
PMID:1107813
Abstract

A mutation affecting the activity of the first enzyme in glutathione biosynthesis in E. coli K 12 has been mapped. The mutant allele called gshA is located by transduction in the pheA-nalB segment. The linkages with pheA and tyrA provide a convenient method for transfer of gshA to other strains and so introduce a very low level of non-protein sulfhydryl groups into the E. coli cells.

摘要

已对影响大肠杆菌K12谷胱甘肽生物合成中第一种酶活性的突变进行了定位。名为gshA的突变等位基因通过转导定位在pheA-nalB区段。与pheA和tyrA的连锁为将gshA转移到其他菌株提供了一种便捷方法,从而将极低水平的非蛋白质巯基引入大肠杆菌细胞。

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