Boussouel N, Mathieu F, Revol-Junelles A M, Millière J B
Laboratoire de Fermentations et Bioconversions Industrielles, Institut National Polytechnique de Lorraine, Ecole Nationale Supérieure d 'Agronomie et des Industries Alimentaires, Vandoeuvre-Les-Nancy Cedex, France.
Int J Food Microbiol. 2000 Nov 1;61(2-3):169-75. doi: 10.1016/s0168-1605(00)00365-2.
Individual or combined effects of nisin (100 or 200 IU/ml) and the lactoperoxidase system (LPS) were analysed against 1 x 10(4) cfu/ml Listeria monocytogenes ATCC 15313 cells in skim milk, at 25 degrees C for 15 days. Nisin induced an immediate bactericidal effect and LPS a 48 h bacteriostatic phase which in both cases was followed by re-growth of L. monocytogenes. LPS and nisin added together at t0 showed a synergistic and lasting bactericidal effect which after 8 days and until 15 days resulted in no detectable cells in 1 ml of milk. When LPS was added to cells already in contact with 100 or 200 IU/ml nisin for a period of 4 h, the inhibitory activity was enhanced with no L. monocytogenes detectable after 72 or 48 h, respectively, and until 15 days. When LPS was added after 12 h, the nisin bactericidal phase was followed by re-growth. When nisin, 100 or 200 UI/ml, was added to cells already in contact with LPS over 24 h, L. monocytogenes was not detectable after 196 and 244 h, respectively, without any re-growth. For nisin addition after 72 h, cell counts were 8 log10 cycles lower than in the control milk after 196 h, but population levels were similar to the control within 15 days. The best combination to inhibit L. monocytogenes ATCC 15313 was nisin present at t0 followed by the LPS addition 4 h later, when the maximum inhibitory effect of nisin was reached.
在25℃下，对脱脂牛奶中1×10⁴ cfu/ml的单核细胞增生李斯特菌ATCC 15313细胞分析了乳酸链球菌素（100或200 IU/ml）和乳过氧化物酶系统（LPS）的单独或联合作用，持续15天。乳酸链球菌素产生即时杀菌作用，而LPS产生48小时抑菌期，在这两种情况下随后均出现单核细胞增生李斯特菌的再生长。在t0时一起添加LPS和乳酸链球菌素显示出协同且持久的杀菌作用，8天后直至15天，1 ml牛奶中未检测到细胞。当将LPS添加到已经与100或200 IU/ml乳酸链球菌素接触4小时的细胞中时，抑制活性增强，分别在72或48小时后直至15天未检测到单核细胞增生李斯特菌。当在12小时后添加LPS时，乳酸链球菌素杀菌期后出现再生长。当将100或200 UI/ml的乳酸链球菌素添加到已经与LPS接触24小时的细胞中时，分别在196和244小时后未检测到单核细胞增生李斯特菌，且没有任何再生长。对于在72小时后添加乳酸链球菌素，196小时后细胞计数比对照牛奶低8个对数周期，但在15天内种群水平与对照相似。抑制单核细胞增生李斯特菌ATCC 15313的最佳组合是在t0时存在乳酸链球菌素，然后在4小时后添加LPS，此时达到乳酸链球菌素的最大抑制作用。
