Usuki S, Kondoh K, Kubo T
Department of Obstetrics and Gynecology, Institute of Clinical Medicine, University of Tsukuba, Tennodai, Ibaraki, Japan.
J Cardiovasc Pharmacol. 2000 Nov;36(5 Suppl 1):S421-7.
In order to investigate the endothelin (ET) levels and their relationship to various hormones during the menstrual cycle, we measured endothelin-1, -2 and -3 (ET-1, -2 and -3), big ET-1 and big ET-3 levels in 27 normally cycling women (mean age 27 years). Simultaneous determination of luteinizing hormone-releasing hormone (LH-RH), luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin, progesterone, 17alpha-hydroxyprogesterone (17-OHP), estrone, 17beta-estradiol, delta4-androstenedione, testosterone, active renin, angiotensin-II (A-II) and atrial natriuretic peptide (ANP) in blood, and LH, estrone and 17beta-estradiol and pregnanediol levels in urine was made in the same 27 women. The levels of ET-2 in plasma were found to be 20% of those of ET-1. In addition, ET-1 levels were measured in the endometrium of the normal uterus. Plasma ET-1 and ET-3 levels fluctuated during the menstrual cycle, with a peak at the luteal phase, but showed only a negative relationship (p < 0.01) to each other at the menstrual phase, whereas big ET-1 and big ET-3 levels showed no significant changes. Plasma ET-1 and ET-3 levels showed no significant relationship to the big ETs. As for the relationship to other hormones, plasma ET-3 had a negative relationship (p < 0.01) to prolactin and a positive correlation (p < 0.01) with ANP during the entire menstrual cycle. Plasma ET-1 and ET-3 showed a partial positive or negative correlation to LH, FSH, prolactin and ANP levels, depending upon the cycle phase, whereas plasma ETs and big ETs were unrelated to other hormones in the blood, and LH, estrone, 17beta-estradiol and pregnanediol in the urine throughout the menstrual cycle. At each menstrual stage, plasma ET-3 levels were more significantly related to LH, FSH, prolactin and ANP than ET-1, indicating a closer relationship between ET-3 and these circulating hormones during the menstrual cycle. The ET-1 level showed no significant change in the endometrium during the menstrual cycle.
为了研究月经周期中内皮素（ET）水平及其与各种激素的关系，我们检测了27名月经周期正常的女性（平均年龄27岁）体内内皮素-1、-2和-3（ET-1、-2和-3）、大内皮素-1和大内皮素-3的水平。同时测定了这27名女性血液中的促黄体生成激素释放激素（LH-RH）、促黄体生成激素（LH）、促卵泡生成激素（FSH）、催乳素、孕酮、17α-羟孕酮（17-OHP）、雌酮、17β-雌二醇、Δ4-雄烯二酮、睾酮、活性肾素、血管紧张素-II（A-II）和心钠素（ANP），以及尿液中的LH、雌酮、17β-雌二醇和孕二醇水平。发现血浆中ET-2的水平是ET-1水平的20%。此外，还检测了正常子宫内膜中的ET-1水平。血浆ET-1和ET-3水平在月经周期中波动，在黄体期达到峰值，但在月经期两者仅呈负相关（p<0.01），而大内皮素-1和大内皮素-3水平无明显变化。血浆ET-1和ET-3水平与大内皮素无显著关系。至于与其他激素的关系，在整个月经周期中，血浆ET-3与催乳素呈负相关（p<0.01），与ANP呈正相关（p<0.01）。血浆ET-1和ET-3与LH、FSH、催乳素和ANP水平的相关性取决于月经周期阶段，呈部分正相关或负相关，而血浆ET和大内皮素与血液中的其他激素以及整个月经周期尿液中的LH、雌酮、17β-雌二醇和孕二醇均无关联。在每个月经阶段，血浆ET-3水平与LH、FSH、催乳素和ANP的相关性均比ET-1更显著，表明在月经周期中ET-3与这些循环激素的关系更为密切。月经周期中子宫内膜的ET-1水平无明显变化。
