Morinaga N, Shitara Y, Yanagita Y, Koida T, Kimura M, Asao T, Kimijima I, Takenoshita S, Hirota T, Saya H, Kuwano H
Department of Surgery I, Faculty of Medicine, Gunma University, Maebashi, Gunma 371-8511, Japan.
Int J Oncol. 2000 Dec;17(6):1125-9. doi: 10.3892/ijo.17.6.1125.
Loss of heterozygosity (LOH) on chromosome 6q is often observed in breast cancer, suggesting the existence of a putative tumor suppressor. Recently, a human homolog of the Drosophila warts tumor suppressor gene, h-warts/LATS1, was identified and mapped at chromosome 6q24-25.1. Mutation analysis of the h-warts/LATS1 was performed using 25 breast cancer tissues by RT-PCR SSCP analysis. Although LOH of the h-warts/LATS1 was found in one patient, no mutations were found. Two polymorphisms were found, but neither of them caused amino acid substitutions. Further investigations are necessary to elucidate the role of the h-warts/LATS1 gene in the carcinogenesis of breast cancer.
6号染色体长臂杂合性缺失（LOH）在乳腺癌中经常被观察到，这表明存在一个假定的肿瘤抑制基因。最近，果蝇warts肿瘤抑制基因的人类同源物h-warts/LATS1被鉴定出来，并定位在6号染色体长臂24-25.1区域。通过逆转录聚合酶链反应-单链构象多态性分析（RT-PCR SSCP分析），对25个乳腺癌组织进行了h-warts/LATS1的突变分析。虽然在一名患者中发现了h-warts/LATS1的杂合性缺失，但未发现突变。发现了两个多态性，但它们都没有导致氨基酸替换。需要进一步研究以阐明h-warts/LATS1基因在乳腺癌致癌过程中的作用。
