Deng Jihong, Zhang Wen, Liu Shuangyue, An Hongmei, Tan Lu, Ma Lisha
Department of Gynecology, Kunming Maternity and Child Care Hospital, Kunming, Yunnan 650000, P.R. China.
Mol Med Rep. 2017 Apr;15(4):1654-1660. doi: 10.3892/mmr.2017.6180. Epub 2017 Feb 8.
Loss of large tumor suppressor kinase 1 (LATS1)Y has been implicated in numerous types of human cancer. However, its involvement in human cervical cancer remains to be elucidated. The present study aimed to investigate the clinical significance and biological characteristics of LATS1 in human cervical cancer. The present study investigated the protein expression levels of LATS1 in tissues from 80 cases of cervical cancer using immunohistochemistry and demonstrated that LATS1 was downregulated in 45% (36/80) of cervical cancers. Transfection of LATS1 was performed in the SiHa cell line and LATS1 siRNA knockdown was performed in the Caski cell line. MTT assay and Matrigel invasion assay indicated that LATS1 overexpression inhibited cell proliferation and invasion. LATS1 overexpression upregulated p27 expression, and downregulated the expression of cyclin E and matrix metalloproteinase 9. In addition, LATS1 overexpression stimulated yes‑associated protein 1 (YAP) phosphorylation. Depletion of LATS1 in Caski cells resulted in the opposite effects. The current study demonstrated that LATS1 was downregulated in cervical cancer and may suppress cell growth and invasion through regulating the expression of cyclin E, p27, MMP9 and YAP.
大肿瘤抑制激酶1（LATS1）缺失与多种人类癌症有关。然而，其在人类宫颈癌中的作用仍有待阐明。本研究旨在探讨LATS1在人类宫颈癌中的临床意义和生物学特性。本研究采用免疫组织化学方法检测了80例宫颈癌组织中LATS1的蛋白表达水平，结果显示45%（36/80）的宫颈癌组织中LATS1表达下调。在SiHa细胞系中进行LATS1转染，在Caski细胞系中进行LATS1 siRNA敲低。MTT法和基质胶侵袭试验表明，LATS1过表达抑制细胞增殖和侵袭。LATS1过表达上调p27表达，下调细胞周期蛋白E和基质金属蛋白酶9的表达。此外，LATS1过表达刺激Yes相关蛋白1（YAP）磷酸化。在Caski细胞中敲低LATS1则产生相反的效果。目前的研究表明，LATS1在宫颈癌中表达下调，可能通过调节细胞周期蛋白E、p27、MMP9和YAP的表达来抑制细胞生长和侵袭。
