核酸复性动力学研究：DNA-DNA复性中单链尾的反应活性
Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.
作者信息
Smith M J, Britten R J, Davidson E H
出版信息
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4805-9. doi: 10.1073/pnas.72.12.4805.
Abstract
The reassociation kinetics of Escherichia coli DNA were measured by S1 nuclease resistance and hydroxyapatite binding. While the reaction assayed by hydroxyapatite displays second order kinetics, the S1 nuclease measurements follow a non-second order from, as previously reported by Morrow (Ph.D. Dissertation, Stanford University. 1974). Much of the reaction measured with S1 nuclease occurs between single stranded regions of fragments already bearing duplex structures from previous collisions, and between such regions and totally free single strands. Experimental determinations indicate that the nucleation rate of single stranded regions on fragments also containing duplexes is inhibited by an average factor of 2 to 4.
摘要
通过S1核酸酶抗性和羟基磷灰石结合来测定大肠杆菌DNA的复性动力学。虽然通过羟基磷灰石测定的反应呈现二级动力学，但正如Morrow之前报道的那样（博士论文，斯坦福大学，1974年），S1核酸酶测量结果遵循非二级动力学。用S1核酸酶测量的大部分反应发生在已经因先前碰撞而带有双链结构的片段的单链区域之间，以及这些区域与完全游离的单链之间。实验测定表明，在也含有双链体的片段上单链区域的成核速率平均受到2至4倍的抑制。
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