核酸重缔合动力学研究：描述DNA重缔合的经验方程。
Studies on nucleic acid reassociation kinetics: empirical equations describing DNA reassociation.
作者信息
Britten R J, Davidson E H
出版信息
Proc Natl Acad Sci U S A. 1976 Feb;73(2):415-9. doi: 10.1073/pnas.73.2.415.
Abstract
The rate of appearance of duplex DNA renaturation, measured with single strand specific nuclease, deviates significantly from a second order reaction. Measurements reported in paper I of this series indicate an inhibition in the rate of reassociation of single strand tails on partially reassociated molecules by a factor of at least two. Equations are derived that describe the observed form of reassociation kinetics as measured with hydroxyapatite and with single strand specific nuclease. The free parameter that describes the extent of inhibition of nucleation with single strand tails in these equations has been evaluated by least squares methods and agrees with the experimentally measured value.
摘要
用单链特异性核酸酶测量的双链DNA复性出现速率显著偏离二级反应。本系列论文I中报道的测量结果表明，部分复性分子上单链尾巴的重新缔合速率受到至少两倍的抑制。推导了描述用羟基磷灰石和单链特异性核酸酶测量的复性动力学观察形式的方程。这些方程中描述单链尾巴对成核抑制程度的自由参数已通过最小二乘法进行评估，且与实验测量值相符。
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本文引用的文献
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Kinetics of renaturation of DNA.DNA复性动力学
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Excluded volume effects on the rate of renaturation of DNA.排除体积对DNA复性速率的影响。
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Sequence organization in Xenopus DNA studied by the electron microscope.用电镜研究非洲爪蟾DNA的序列组织。
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4
Studies on nucleic acid reassociation kinetics: reactivity of single-stranded tails in DNA-DNA renaturation.核酸复性动力学研究：DNA-DNA复性中单链尾的反应活性
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