来自酵母嗜杀酵母Yp74L-3的乳清苷-5'-磷酸脱羧酶基因的分子克隆及DNA分析

Molecular cloning and DNA analysis of the orotidine-5'-phosphate decarboxylase gene from the yeast Saccharomyces exiguus Yp74L-3.

作者信息

Hisatomi T, Kodama T, Toba H, Moto-oka M, Watanabe A, Tsuboi M

机构信息

Department of Biotechnology, Faculty of Engineering, Fukuyama University, Gakuen-cho, Fukuyama, Hiroshima 729-0292, Japan.

出版信息

Curr Microbiol. 2000 Dec;41(6):421-4. doi: 10.1007/s002840010161.

DOI:10.1007/s002840010161

PMID:11080392

Abstract

The orotidine-5'-phosphate decarboxylase gene of Saccharomyces exiguus Yp74L-3 was cloned as a DNA fragment complementing a ura4 mutation of this yeast. The coding region of the gene is 807 bp in length, and represents 68.7% similarity to the corresponding gene of S. cerevisiae (URA3). The cloned URA4 gene was shown to be located on the 790-kbp Chromosome (chr) VIII of S. exiguus Yp74L-3. The neighbor-joining phylogenetic tree based on the orotidine-5'-phosphate decarboxylase coding sequences indicates that S. exiguus Yp74L-3 is closely related to Kluyveromyces yeasts, as well as to a S. cerevisiae laboratory strain.

摘要

短小酿酒酵母Yp74L-3的乳清苷-5'-磷酸脱羧酶基因作为一个可互补该酵母ura4突变的DNA片段被克隆。该基因的编码区长度为807 bp，与酿酒酵母（URA3）的相应基因具有68.7%的相似性。克隆的URA4基因位于短小酿酒酵母Yp74L-3的790-kbp VIII号染色体（chr）上。基于乳清苷-5'-磷酸脱羧酶编码序列构建的邻接法系统发育树表明，短小酿酒酵母Yp74L-3与克鲁维酵母以及酿酒酵母实验室菌株密切相关。