Owicki J C
J Biomol Screen. 2000 Oct;5(5):297-306. doi: 10.1177/108705710000500501.
Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct homogeneous assays that are fast, sensitive, and resistant to some significant interferences. Moreover, the assays are relatively inexpensive. This review surveys the peer-reviewed literature on the subject and explores some of the fundamental issues that bear on assay performance.
荧光偏振和各向异性是两种几乎等效的技术，在过去五年中，它们在药物发现的高通量筛选中得到了广泛应用。这些是单标记方法，可用于构建快速、灵敏且能抵抗一些重大干扰的均相分析。此外，这些分析相对便宜。本综述调查了关于该主题的同行评审文献，并探讨了一些与分析性能相关的基本问题。
