Sonoda S, Li H C, Cartier L, Nunez L, Tajima K
Department of Virology, Faculty of Medicine, Kagoshima University, Kagoshima, Japan.
AIDS Res Hum Retroviruses. 2000 Nov 1;16(16):1753-6. doi: 10.1089/08892220050193263.
The worldwide geographic and ethnic clustering of patients with diseases related to human T cell lymphotropic virus type 1 (HTLV-1) may be explained by the natural history of HTLV-1 infection. The genetic characteristics of indigenous people in the Andes are similar to those of the Japanese, and HTLV-1 is generally detected in both groups. To clarify the common origin of HTLV-1 in Asia and the Andes, we analyzed HTLV-1 provirus DNA from Andean mummies about 1500 years old. Two of 104 mummy bone marrow specimens yielded a band of human beta-globin gene DNA 110 base pairs in length, and one of these two produced bands of HTLV-1-pX (open reading frame encoding p(40x), p(27x)) and HTLV-1-LTR (long terminal repeat) gene DNA 159 base pairs and 157 base pairs in length, respectively. The nucleotide sequences of ancient HTLV-1-pX and HTLV-1-LTR clones isolated from mummy bone marrow were similar to those in contemporary Andeans and Japanese, although there was microheterogeneity in the sequences of some mummy DNA clones. This result provides evidence that HTLV-1 was carried with ancient Mongoloids to the Andes before the Colonial era. Analysis of ancient HTLV-1 sequences could be a useful tool for studying the history of human retroviral infection as well as human prehistoric migration.
与人类嗜T细胞病毒1型（HTLV-1）相关疾病患者在全球范围内的地理和种族聚集现象，可能可以用HTLV-1感染的自然史来解释。安第斯地区原住民的基因特征与日本人相似，并且在这两个群体中通常都能检测到HTLV-1。为了阐明亚洲和安第斯地区HTLV-1的共同起源，我们分析了来自约1500年前安第斯木乃伊的HTLV-1前病毒DNA。在104份木乃伊骨髓标本中，有两份产生了长度为110个碱基对的人类β-珠蛋白基因DNA条带，其中一份还分别产生了长度为159个碱基对和157个碱基对的HTLV-1-pX（编码p(40x)、p(27x)的开放阅读框）和HTLV-1-LTR（长末端重复序列）基因DNA条带。从木乃伊骨髓中分离出的古代HTLV-1-pX和HTLV-1-LTR克隆的核苷酸序列与当代安第斯人和日本人的相似，尽管一些木乃伊DNA克隆的序列存在微异质性。这一结果提供了证据，表明HTLV-1在殖民时代之前就随着古代蒙古人种被带到了安第斯地区。分析古代HTLV-1序列可能是研究人类逆转录病毒感染历史以及人类史前迁徙的有用工具。
