在hap1和rox1突变背景下对酿酒酵母203个新基因的转录本分析。

Transcript analysis of 203 novel genes from Saccharomyces cerevisiae in hap1 and rox1 mutant backgrounds.

作者信息

Lombardía L J, Cadahía-Rodríguez J L, Freire-Picos M A, González-Siso M I, Rodríguez-Torres A M, Cerdán M E

机构信息

Departamento de Biología Celular y Molecular, Universidad de La Coruña, Spain.

出版信息

Genome. 2000 Oct;43(5):881-6. doi: 10.1139/g00-049.

DOI:10.1139/g00-049

PMID:11081979

Abstract

Hap1 and Rox1 are transcriptional regulators that bind regulatory sites in the promoters of oxygen-regulated genes in Saccharomyces cerevisiae. Hap1 is a heme-responsive activator of genes induced in aerobic conditions and Rox1 is a repressor of hypoxic genes in aerobic conditions. We have studied transcriptional regulation of a pool of 203 open reading frames (ORFs) from chromosomes IV, VII, and XIV in wild-type, hap1, and rox1 mutant genetic backgrounds in an attempt to extend the family of oxygen and heme regulated genes. Only three ORFs are significantly repressed by Rox1 but they cannot be considered as typical hypoxic genes because they are not overexpressed during hypoxia.

摘要

Hap1和Rox1是转录调节因子，它们结合酿酒酵母中氧调节基因启动子中的调控位点。Hap1是在有氧条件下诱导基因的血红素应答激活因子，而Rox1是有氧条件下低氧基因的阻遏因子。我们研究了野生型、hap1和rox1突变体遗传背景下来自IV、VII和XIV号染色体的203个开放阅读框（ORF）的转录调控，试图扩展氧和血红素调节基因家族。只有三个ORF被Rox1显著抑制，但它们不能被视为典型的低氧基因，因为它们在低氧期间并未过度表达。