Smith L, Novák J, Rocca J, McClung S, Hillman J D, Edison A S
Department of Neuroscience, University of Florida, Gainesville, Florida 32610, USA.
Eur J Biochem. 2000 Dec;267(23):6810-6. doi: 10.1046/j.1432-1033.2000.01777.x.
The primary structure of the Streptococcus mutans lantibiotic mutacin 1140 was elucidated by NMR spectroscopy, mass spectrometry, and chemical sequencing. The structure is in agreement with other closely related lantibiotics, such as epidermin. A novel method was developed in which mutacin 1140 was chemically modified with sodium borohydride followed by ethanethiol, allowing the differentiation of the thioether-containing residues from the dehydrated residues. This double-labeling strategy provides a simple method to reliably identify all modified lantibiotic residues with a minimal amount of material. While NMR spectroscopy is still required to obtain thioether bridging patterns and thus the complete covalent structure, the double-labeling technique, along with mass spectrometry, provides most of the information in a fraction of the time required for a complete NMR analysis. Thus, with these new techniques lantibiotics can be rapidly characterized.
通过核磁共振光谱、质谱和化学测序阐明了变形链球菌羊毛硫抗生素变链菌素1140的一级结构。该结构与其他密切相关的羊毛硫抗生素，如表皮菌素一致。开发了一种新方法，其中用硼氢化钠然后用乙硫醇对变链菌素1140进行化学修饰，从而能够区分含硫醚的残基和脱水残基。这种双标记策略提供了一种简单的方法，能够以最少的材料可靠地鉴定所有修饰的羊毛硫抗生素残基。虽然仍需要核磁共振光谱来获得硫醚桥接模式并从而获得完整的共价结构，但双标记技术与质谱一起，能在完整核磁共振分析所需时间的一小部分内提供大部分信息。因此，利用这些新技术可以快速表征羊毛硫抗生素。
