变链菌素B-Ny266的纯化与结构：一种由变形链球菌产生的新型羊毛硫抗生素。

Purification and structure of mutacin B-Ny266: a new lantibiotic produced by Streptococcus mutans.

作者信息

Mota-Meira M, Lacroix C, LaPointe G, Lavoie M C

机构信息

Centre de Recherche en Sciences et Technologie du Lait (STELA), Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec, Canada.

出版信息

FEBS Lett. 1997 Jun 30;410(2-3):275-9. doi: 10.1016/s0014-5793(97)00425-0.

DOI:10.1016/s0014-5793(97)00425-0

PMID:9237644

Abstract

Mutacins are bactericidal substances of proteinaceous nature produced by Streptococcus mutans. Lantibiotics are antibacterial substances containing post-translationally modified amino acids such as lanthionine. Mutacin B-Ny266 was purified from the cell pellet of S. mutans strain Ny266 by ethanol extraction at pH 2.0 followed by reversed-phase chromatography (Sep-Pak cartridge) and by HPLC on a C18 column. The mean purification factor was 3240 +/- 81 and the mean yield was 1.0 +/- 0.1%. Molecular mass of mutacin B-Ny266 as determined by mass spectroscopy is 2270.29 +/- 0.21 Da. The amino acid sequence of the purified active fraction was obtained by Edman degradation after treatment with alkaline ethanethiol. Twenty-one amino acids were detected in this analysis. Mutacin B-Ny266 belongs to the type A lantibiotics. The proposed sequence is: F-K-A-W-U-F-A-Abu-P-G-A-A-K-O-G-A-F-N-U-Y-A. The molecule differs from that of epidermin/staphylococcin 1580 and gallidermin at positions 1, 2, 4, 5 and 6.

摘要

变链菌素是变形链球菌产生的具有蛋白质性质的杀菌物质。羊毛硫抗生素是一类含有翻译后修饰氨基酸（如羊毛硫氨酸）的抗菌物质。变链菌素B-Ny266是通过在pH 2.0条件下用乙醇从变形链球菌菌株Ny266的细胞沉淀中提取，然后进行反相色谱法（Sep-Pak柱）以及在C18柱上进行高效液相色谱法纯化得到的。平均纯化因子为3240±81，平均产率为1.0±0.1%。通过质谱测定，变链菌素B-Ny266的分子量为2270.29±0.21道尔顿。纯化的活性组分的氨基酸序列在用碱性乙硫醇处理后通过埃德曼降解法获得。在此分析中检测到21个氨基酸。变链菌素B-Ny266属于A型羊毛硫抗生素。推测的序列为：F-K-A-W-U-F-A-Abu-P-G-A-A-K-O-G-A-F-N-U-Y-A。该分子在第1、2、4、5和6位与表皮菌素/葡萄球菌素1580和加里德菌素不同。