Skrzypczak-Pietraszek E, Hensel A
Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, Kraków, Poland.
Pharmazie. 2000 Oct;55(10):768-71.
For comparison of the water-soluble polysaccharides from Melittis melissophyllum L. herb with that produced by Melittis callus cultures the polymeric carbohydrates were extracted from both sources, fractionated by IEC and GPC and the respective fractions analysed concerning sugar composition and linkage characteristics. The dominant structures found in all fractions isolated from herb material and callus were type-II arabinogalactans with a (1-->3)-galactose backbone and arabinose-galactose side chains. No significant differences were found between herb and callus polysaccharides. To optimize the Melittis cell culture systems the culture media were varried systematically. A modified Murashige-Skoog (MS) medium, supplemented with GA3, NAA and BAP was found to be most suitable for large-scale production of callus material.
为了比较蜜蜂花（Melittis melissophyllum L.）全草中的水溶性多糖与蜜蜂花愈伤组织培养物产生的水溶性多糖，从这两种来源提取聚合碳水化合物，通过离子交换色谱（IEC）和凝胶渗透色谱（GPC）进行分级分离，并对各组分的糖组成和连接特性进行分析。从全草材料和愈伤组织中分离得到的所有组分中发现的主要结构是具有（1→3）-半乳糖主链和阿拉伯糖-半乳糖侧链的II型阿拉伯半乳聚糖。在全草和愈伤组织多糖之间未发现显著差异。为了优化蜜蜂花细胞培养系统，系统地改变了培养基。发现一种添加了赤霉素（GA3）、萘乙酸（NAA）和苄氨基嘌呤（BAP）的改良Murashige-Skoog（MS）培养基最适合大规模生产愈伤组织材料。
