[高效液相色谱法测定岩青兰药材中木犀草素-7-O-糖苷的含量]
[Determination of luteolin-7-O-glycoside in the herb of Dracocephalum rupestra by HPLC].
作者信息
Ren Dong-mei, Lou Hong-xiang, Ma Bin, Ji Mei
机构信息
School of Pharmacy, Shandong University, Jinan 250012, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2004 Sep;29(9):860-2.
OBJECTIVE
To develop a method for determination of luteolin-7-O-glycoside in the roots, stems, leafs, flowers and aerial parts of Dracocephalum rupestra sampled in different seasons.
METHOD
The samples were analyzed on an phenomenex C18 column, with mobile phase of methanol-acetonitrile-0.4% phosphoric acid (30:8:62) at flow rate 1.0 mL x min(-1) and detection at wavelength of 350 nm.
RESULT
The content of luteolin-7-O-glycoside in different parts of D. rupestra was different maximum in leaves, while minimum in stems. Luteolin-7-O-glycoside in D. rupestra sampled before blossoming was the highest.
CONCLUSION
The method simple, accurate and suitable for the quality evaluation of this plant medicine.
目的
建立不同季节采集的岩青兰根、茎、叶、花及地上部分中木犀草素-7-O-糖苷的测定方法。
方法
采用phenomenex C18柱分析样品，流动相为甲醇-乙腈-0.4%磷酸（30:8:62），流速1.0 mL·min⁻¹，检测波长350 nm。
结果
岩青兰不同部位木犀草素-7-O-糖苷含量不同，叶中含量最高，茎中含量最低。开花前采集的岩青兰中木犀草素-7-O-糖苷含量最高。
结论
该方法简便、准确，适用于该植物药的质量评价。
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