Cox C D, Parker J
J Clin Microbiol. 1979 Apr;9(4):479-84. doi: 10.1128/jcm.9.4.479-484.1979.
A grapelike odor is often of diagnostic importance in detecting the growth of Pseudomonas aeruginosa in culture and in burn wounds. The compound responsible for the odor has been identified as 2-aminoacetophenone by mass spectroscopy. Although the grape odor is sometimes difficult to detect in culture media, gas chromatographic, fluorometric, and colorimetric methods can be utilized to assay 2-aminoacetophenone production in a variety of media. Its synthesis occurs relatively early in the growth cycle. It has proved easy and convenient to detect 2-aminoacetophenone excretion by P. aeruginosa after 24 h of incubation on blood agar plates employing a fluorometric assay of ether extracts of the agar medium.
葡萄样气味在检测铜绿假单胞菌在培养物及烧伤创面中的生长情况时通常具有诊断意义。通过质谱分析已确定产生该气味的化合物为2-氨基苯乙酮。尽管在培养基中有时难以检测到葡萄样气味，但气相色谱法、荧光法和比色法可用于测定多种培养基中2-氨基苯乙酮的产生情况。其合成在生长周期中相对较早发生。事实证明，在血琼脂平板上孵育24小时后，采用琼脂培养基乙醚提取物的荧光测定法来检测铜绿假单胞菌分泌的2-氨基苯乙酮既简便又容易。
