Domitrz J, Wołczyński S, Syrewicz M, Kuczyński W, Szamatowicz J, Grochowski D, Szamatowicz M
Zakład Endokrynologii Ginekologicznej AM w Białymstoku.
Ginekol Pol. 2000 Sep;71(9):1047-52.
The study was conducted on the patients who had attempted and failed previous IVF procedures. The aim of this study was to determinate effectiveness sequential culture of embryos to blastocyst and assisted hatching by zona pellucida digestation by pronase. In this group the pregnancy rate was 24.1% compared to control 17.8%. The implantation rate was higher in study group than control one. It suggests that the culture embryos to balstocyste stage and assisted hatching by digestation by pronase is safe and effective mode of treatment of infertile couple after failed attempts IVF ET.
该研究针对此前进行体外受精（IVF）程序但失败的患者展开。本研究的目的是确定将胚胎序贯培养至囊胚以及通过用链霉蛋白酶消化透明带来进行辅助孵化的有效性。在该组中，妊娠率为24.1%，而对照组为17.8%。研究组的着床率高于对照组。这表明将胚胎培养至囊胚阶段并通过链霉蛋白酶消化进行辅助孵化，是体外受精 - 胚胎移植（IVF - ET）尝试失败后不孕夫妇的一种安全有效的治疗方式。
