Aebersold R, Rist B, Gygi S P
Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
Ann N Y Acad Sci. 2000;919:33-47. doi: 10.1111/j.1749-6632.2000.tb06865.x.
With the completion of a rapidly increasing number of complete genomic sequences, much attention is currently focused on how the information contained in sequence databases might be interpreted in terms of the structure, function, and control of biological systems. Quantitative proteome analysis, the global analysis of protein expression, has been proposed as a method to study steady-state gene expression and perturbation-induced changes. Here, we discuss the rationale for quantitative proteome analysis, highlight the limitations in the current standard technology, and introduce a new experimental approach to quantitative proteome analysis.
随着越来越多完整基因组序列的完成，目前人们的注意力大多集中在如何根据生物系统的结构、功能和调控来解读序列数据库中包含的信息。定量蛋白质组分析，即蛋白质表达的全局分析，已被提议作为一种研究稳态基因表达和扰动诱导变化的方法。在此，我们讨论定量蛋白质组分析的基本原理，强调当前标准技术的局限性，并介绍一种新的定量蛋白质组分析实验方法。
