大麻素对正常人星形胶质细胞作用的基因组和蛋白质组分析。
Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes.
作者信息
Bindukumar B, Mahajan S D, Reynolds J L, Hu Z, Sykes D E, Aalinkeel R, Schwartz S A
机构信息
Department of Medicine, Division of Allergy, Immunology, and Rheumatology, Buffalo General Hospital, University at Buffalo, State University of NY, Kaleida Health, 100 High Street, Buffalo, NY 14203, USA.
出版信息
Brain Res. 2008 Jan 29;1191:1-11. doi: 10.1016/j.brainres.2007.10.062. Epub 2007 Nov 1.
Delta-9-tetrahydrocannabinol (Delta(9)-THC), the main psychoactive component of marijuana, is known to dysregulate various immune responses. Cannabinoid (CB)-1 and -2 receptors are expressed mainly on cells of the central nervous system (CNS) and the immune system. The CNS is the primary target of cannabinoids and astrocytes are known to play a role in various immune responses. Thus we undertook this investigation to determine the global molecular effects of cannabinoids on normal human astrocytes (NHA) using genomic and proteomic analyses. NHA were treated with Delta(9)-THC and assayed using gene microarrays and two-dimensional (2D) difference gel electrophoresis (DIGE) coupled with mass spectrometry (MS) to elucidate their genomic and proteomic profiles respectively. Our results show that the expression of more than 20 translated protein gene products from NHA was differentially dysregulated by treatment with Delta(9)-THC compared to untreated, control NHA.
9-四氢大麻酚（Δ9-THC）是大麻的主要精神活性成分，已知其会扰乱各种免疫反应。大麻素（CB）-1和-2受体主要在中枢神经系统（CNS）和免疫系统的细胞上表达。中枢神经系统是大麻素的主要作用靶点，已知星形胶质细胞在各种免疫反应中发挥作用。因此，我们进行了这项研究，通过基因组和蛋白质组分析来确定大麻素对正常人星形胶质细胞（NHA）的整体分子效应。用Δ9-THC处理NHA，并分别使用基因微阵列和二维（2D）差异凝胶电泳（DIGE）结合质谱（MS）进行检测，以阐明它们的基因组和蛋白质组图谱。我们的结果表明，与未处理的对照NHA相比，用Δ9-THC处理后，NHA中20多种已翻译的蛋白质基因产物的表达出现了差异失调。
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