有机阳离子转运体rOCT1和rOCT2在大鼠肾近端小管基底外侧膜中的差异定位。
Differential localization of organic cation transporters rOCT1 and rOCT2 in the basolateral membrane of rat kidney proximal tubules.
作者信息
Sugawara-Yokoo M, Urakami Y, Koyama H, Fujikura K, Masuda S, Saito H, Naruse T, Inui K, Takata K
机构信息
Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.
出版信息
Histochem Cell Biol. 2000 Sep;114(3):175-80. doi: 10.1007/s004180000186.
Organic cation transporters play an important role in the secretion of cationic drugs as well as endogenous cationic metabolites in the renal tubules. Immunoblotting showed the presence of organic cation transporter proteins, rOCT1 and rOCT2, in the rat kidney. By immunofluorescence microscopy, rOCT1 was shown to be concentrated in the proximal tubules in the renal cortex. rOCT2, on the other hand, was rich in the proximal tubules in the outer stripe of the outer medulla. Confocal microscopy revealed that both rOCT1 and rOCT2 were localized to the basolateral membranes of these tubule cells. These findings directly show that rOCT1 and rOCT2 are basolateral membrane proteins and are differentially distributed along the proximal tubules.
有机阳离子转运体在肾小管中阳离子药物以及内源性阳离子代谢产物的分泌过程中发挥着重要作用。免疫印迹法显示大鼠肾脏中存在有机阳离子转运体蛋白rOCT1和rOCT2。通过免疫荧光显微镜观察，rOCT1集中在肾皮质的近端小管中。另一方面，rOCT2在外髓质外带的近端小管中含量丰富。共聚焦显微镜显示rOCT1和rOCT2都定位于这些小管细胞的基底外侧膜。这些发现直接表明rOCT1和rOCT2是基底外侧膜蛋白，并且沿近端小管呈差异分布。
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