脑脊液中的寡克隆区带：等电聚焦、琼脂糖凝胶电泳及IgG指数的比较研究

Oligoclonal bands in cerebrospinal fluid: a comparative study of isoelectric focusing, agarose gel electrophoresis and IgG index.

作者信息

Lunding J, Midgard R, Vedeler C A

机构信息

Department of Neurology, University of Bergen, Norway.

出版信息

Acta Neurol Scand. 2000 Nov;102(5):322-5. doi: 10.1034/j.1600-0404.2000.102005322.x.

DOI:10.1034/j.1600-0404.2000.102005322.x

PMID:11083510

Abstract

OBJECTIVE

To compare the sensitivity and specificity of isoelectric focusing (IEF) with immunofixation, agarose gel electrophoresis (AGE) and the IgG index in detecting intrathecally synthesized IgG in multiple sclerosis (MS) and in other nervous system disorders.

MATERIALS AND METHODS

Cerebrospinal fluid (CSF) and serum from 147 patients with various nervous system diseases, 20 of whom had MS, were compared with IEF, AGE and the IgG index.

RESULTS

CSF-restricted oligoclonal bands (OCB) were found in 20 of 20 patients with MS using IEF and in 9 of 20 using AGE. OCB were found in 12 patients with other nervous system disorders (OND) using IEF and 4 using AGE. The mean IgG index was 0.50 in OND and 0.96 in MS (P< 0.0001). Of 20 MS patients, 9 had an IgG index above the defined cut-off value of 0.72.

CONCLUSIONS

IEF is about twice as sensitive as AGE in detecting OCB in MS. IEF is also far superior to the IgG index in determining intrathecal IgG synthesis.

摘要

目的

比较等电聚焦（IEF）与免疫固定、琼脂糖凝胶电泳（AGE）以及IgG指数在检测多发性硬化症（MS）和其他神经系统疾病中鞘内合成IgG时的敏感性和特异性。

材料与方法

对147例患有各种神经系统疾病的患者（其中20例为MS患者）的脑脊液（CSF）和血清进行IEF、AGE及IgG指数的比较。

结果

使用IEF法，20例MS患者中有20例发现脑脊液限制性寡克隆带（OCB），使用AGE法的20例患者中有9例发现OCB。使用IEF法，12例其他神经系统疾病（OND）患者发现OCB，使用AGE法的有4例。OND患者的平均IgG指数为0.50，MS患者为0.96（P<0.0001）。20例MS患者中，9例的IgG指数高于定义的临界值0.72。