Mehta P D, Patrick B A, Wisniewski H M
J Clin Lab Immunol. 1981 Jul;6(1):17-22.
CSF from 40 MS patients and 30 individuals with other neurologic diseases were analyzed using isoelectric focusing (IEF) in polyacrylamide gel method. The IgG bands were identified by immunofixation using specific goat anti-human IgG serum. CSF from 28 of 40 MS patients (95%) showed 10-15 distinct oligoclonal bands with different isoelectric points and varying intensities. In contrast, CSF from patients with other neurologic diseases showed a number of diffuse IgG bands in IEF after immunofixation. CSF from 30 of 40 MS patients (75%) showed 1-4 oligoclonal IgG bands in agarose gel electrophoresis (PANAGEL), however, the resolution of bands was generally poor. When 34 coded CSF specimens were analyzed in the IEF and immunofixation procedures, 15 of 17 MS patients (88%) showed distinct oligoclonal IgG bands. Thus, IEF in combination with immunofixation is a superior method for detecting oligoclonal bands in CSF and provides a more valuable tool for the diagnosis of MS. The results also showed that a number of distinct individual oligoclonal IgG bands of MS CSF were of either kappa-type or lambda-type, suggesting that these bands are monoclonal. IgG isolated from 12 of 17 MS sera (70%) showed oligoclonal bands in IEF partly similar to those seen in CSF in the pH region 8.5-9.3; whereas, those from other neurologic diseases showed diffuse bands.
采用聚丙烯酰胺凝胶等电聚焦（IEF）法对40例多发性硬化症（MS）患者和30例患有其他神经系统疾病的个体的脑脊液进行分析。使用特异性山羊抗人IgG血清通过免疫固定鉴定IgG条带。40例MS患者中有28例（95%）的脑脊液显示出10 - 15条具有不同等电点和不同强度的明显寡克隆条带。相比之下，患有其他神经系统疾病患者的脑脊液在免疫固定后的IEF中显示出许多弥散的IgG条带。40例MS患者中有30例（75%）的脑脊液在琼脂糖凝胶电泳（PANAGEL）中显示出1 - 4条寡克隆IgG条带，然而，条带的分辨率通常较差。当对34份编码的脑脊液标本进行IEF和免疫固定程序分析时，17例MS患者中有15例（88%）显示出明显的寡克隆IgG条带。因此，IEF与免疫固定相结合是检测脑脊液中寡克隆条带的一种优越方法，为MS的诊断提供了更有价值的工具。结果还表明，MS脑脊液中许多明显的个体寡克隆IgG条带要么是κ型要么是λ型，表明这些条带是单克隆的。从17例MS血清中的12例（70%）分离出的IgG在IEF中显示出寡克隆条带，部分类似于在pH值8.5 - 9.3区域脑脊液中所见的条带；而来自其他神经系统疾病的血清则显示出弥散条带。
