Weingart C L, Mobberley-Schuman P S, Hewlett E L, Gray M C, Weiss A A
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267-0524, USA.
Infect Immun. 2000 Dec;68(12):7152-5. doi: 10.1128/IAI.68.12.7152-7155.2000.
A previous study showed that opsonization with human immune serum could either promote or antagonize phagocytosis of Bordetella pertussis by human neutrophils depending on whether the bacteria expressed adenylate cyclase toxin. Opsonization of the wild-type strain inhibited phagocytosis relative to unopsonized controls. In contrast, mutants lacking adenylate cyclase toxin were efficiently phagocytosed when opsonized with human immune serum. In this study, we examined opsonization in the presence or absence of monoclonal antibodies to adenylate cyclase toxin. Addition of neutralizing monoclonal antibodies to adenylate cyclase toxin converted a serum that previously inhibited both attachment and phagocytosis of the wild-type strain to one that increased both attachment and phagocytosis compared to the no-serum control. Monoclonal antibodies that recognize the adenylate cyclase toxin but fail to neutralize activity were without effect. These results suggest that adenylate cyclase toxin inhibits both Fc receptor-mediated attachment and phagocytosis of B. pertussis by neutrophils.
先前的一项研究表明，用人免疫血清进行调理作用，根据细菌是否表达腺苷酸环化酶毒素，可促进或拮抗人中性粒细胞对百日咳博德特氏菌的吞噬作用。相对于未调理的对照，野生型菌株的调理作用抑制了吞噬作用。相反，缺乏腺苷酸环化酶毒素的突变体在用人类免疫血清调理时能被有效吞噬。在本研究中，我们检测了在有或没有针对腺苷酸环化酶毒素的单克隆抗体存在的情况下的调理作用。添加针对腺苷酸环化酶毒素的中和性单克隆抗体，使一种先前抑制野生型菌株附着和吞噬作用的血清，转变为与无血清对照相比增加了附着和吞噬作用的血清。识别腺苷酸环化酶毒素但不中和其活性的单克隆抗体则没有效果。这些结果表明，腺苷酸环化酶毒素抑制中性粒细胞对百日咳博德特氏菌的Fc受体介导的附着和吞噬作用。
