Galgiani J N, Hewlett E L, Friedman R L
Department of Internal Medicine, University of Arizona College of Medicine, Tucson 85724.
Infect Immun. 1988 Apr;56(4):751-5. doi: 10.1128/iai.56.4.751-755.1988.
Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated inhibition of N-acetylglucosamine incorporation by arthroconidia of Coccidioides immitis in a dose-dependent fashion but had no effect on neutrophil phagocytosis of Candida glabrata and only a slight inhibitory effect on arthroconidial attachment. Neither purified pertussis toxin nor extracts from Bordetella mutants lacking the adenylate cyclase toxin affected neutrophil-mediated inhibition of arthroconidial N-acetylglucosamine incorporation. These studies indicate that adenylate cyclase toxin, alone or in concert with other B. pertussis-elaborated toxins, blocks neutrophil inhibition of arthroconidia, primarily by affecting neutrophil responses other than attachment or phagocytosis.
含有腺苷酸环化酶毒素的百日咳博德特氏菌提取物可使人类中性粒细胞环磷酸腺苷（cAMP）水平大幅升高，并抑制其氧化爆发，这可通过鲁米诺增强化学发光和超氧化物释放反映出来。含有腺苷酸环化酶毒素的提取物以剂量依赖的方式阻断中性粒细胞介导的对粗球孢子菌关节孢子摄取N-乙酰葡糖胺的抑制作用，但对光滑念珠菌的中性粒细胞吞噬作用没有影响，对关节孢子附着仅有轻微抑制作用。纯化的百日咳毒素和缺乏腺苷酸环化酶毒素的百日咳博德特氏菌突变体提取物均不影响中性粒细胞介导的对关节孢子摄取N-乙酰葡糖胺的抑制作用。这些研究表明，腺苷酸环化酶毒素单独或与百日咳博德特氏菌产生的其他毒素协同作用，主要通过影响中性粒细胞除附着或吞噬作用之外的反应来阻断中性粒细胞对关节孢子的抑制作用。
