Tanaka E, Miyawaki Y, Tanaka M, Watanabe M, Lee K, del Pozo R, Tanne K
Department of Orthodontics, Hiroshima University School of Dentistry, 1-2-3 Kasumi, Minami-ku, 734-8553, Hiroshima, Japan.
Arch Oral Biol. 2000 Dec;45(12):1049-57. doi: 10.1016/s0003-9969(00)00083-2.
The aim was to investigate the direct contribution of mechanically induced stress to synthesized type III collagen in the interparietal suture. Twenty 4-week-old male rats were used as experimental animals. An activated expansion spring was placed across the interparietal suture. After expansion, the parietal bones including the suture were dissected and immunostained for type I and III collagens. Also, a three-dimensional model of the interparietal suture was developed to analyze stress during expansion. The localization of type III collagen in the suture was dependent upon the duration of expansion. Although the control suture exhibited no or little reactivity for type III collagen, it was observed as early as 15 h of expansion in the cambial layers, and was distributed throughout the suture at 50 h of expansion. The time-dependent distribution of type III collagen was fully consistent with that of tensile stresses calculated by the use of a three-dimensional finite-element model. It was concluded that the increases in tensile stress are associated with the synthesis of type III collagen in the suture.
目的是研究机械诱导应力对顶间缝中合成的III型胶原蛋白的直接作用。选用20只4周龄雄性大鼠作为实验动物。将一个激活的扩张弹簧横跨顶间缝放置。扩张后，将包括缝线在内的顶骨进行解剖，并对I型和III型胶原蛋白进行免疫染色。此外，建立了顶间缝的三维模型以分析扩张过程中的应力。III型胶原蛋白在缝线中的定位取决于扩张的持续时间。虽然对照缝线对III型胶原蛋白无反应或反应微弱，但在形成层中最早在扩张15小时时就观察到了它，并且在扩张50小时时分布于整个缝线。III型胶原蛋白随时间的分布与使用三维有限元模型计算出的拉应力分布完全一致。得出的结论是，拉应力的增加与缝线中III型胶原蛋白的合成有关。
