Aalfs J D, Kingston R E
Dept of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Trends Biochem Sci. 2000 Nov;25(11):548-55. doi: 10.1016/s0968-0004(00)01689-3.
The regulated alteration of chromatin structure, termed 'chromatin remodeling', can be accomplished by covalent modification of histones or by the action of ATP-dependent remodeling complexes. A variety of mechanisms can be used to remodel chromatin; some act locally on a single nucleosome and others act more broadly. It is critical to establish a direct connection between the remodeling events observed in vivo and the mechanistic capabilities of remodeling complexes in vitro.
染色质结构的调控性改变,即“染色质重塑”,可通过组蛋白的共价修饰或ATP依赖的重塑复合物的作用来实现。多种机制可用于重塑染色质;一些机制在单个核小体上局部起作用,而其他机制作用范围更广。在体内观察到的重塑事件与体外重塑复合物的机制能力之间建立直接联系至关重要。