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[两种分离小鼠T淋巴细胞和B淋巴细胞方法的比较特征]

[Comparative characteristics of 2 methods of separating mouse T- and B-lymphocytes].

作者信息

Iudin V M, Fedorovskaia M I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1975 Oct(10):115-9.

PMID:1108512
Abstract

The results of separation of T- and B-cells of the C3H/Sn and AKR/j micr lymph nodes with AKR-anti-theta3H and C3H-thetaAKR isosera and cellular immunosorbents containing the antigen-antibody complexes were analyzed in this work. The strain differences in the theta+-cells (T-lymphocytes) level were demonstrated in the untreated mice of the noted strains. Populations enriched by B-lymphocytes under different methods of separation differed somewhat in composition. The cellular immunosorption had many advantages in comparison with the cytotoxic-immune sera used for the T- and B-cell separation.

摘要

本研究分析了用AKR抗θ3H血清和C3H-θAKR同种血清以及含有抗原-抗体复合物的细胞免疫吸附剂分离C3H/Sn和AKR/j小鼠淋巴结中T细胞和B细胞的结果。在上述品系未经处理的小鼠中,已证明θ+细胞(T淋巴细胞)水平存在品系差异。通过不同分离方法富集的B淋巴细胞群体在组成上略有不同。与用于T细胞和B细胞分离的细胞毒性免疫血清相比,细胞免疫吸附具有许多优点。

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