Mair G R, Maule A G, Day T A, Halton D W
Parasitology Research Group, School of Biology and Biochemistry, Medical Biology Centre, The Queen's University of Belfast, UK.
Parasitology. 2000 Aug;121 ( Pt 2):163-70. doi: 10.1017/s0031182099006174.
Using the filamentous actin marker, FITC-conjugated phalloidin, the major muscle systems of adult male and female schistosomes have been examined. The body wall musculature comprises an outer sheath of circular fibres, within which there is a compact layer of short, spindle-shaped longitudinal fibres and a lattice-like arrangement of inner diagonal fibres. Within the oral sucker and acetabulum 3 fibre types, circular, radial and longitudinal can be distinguished. The wall of the oesophagus is lined by a grid-like array of circular and longitudinal fibres, whereas the walls of the intestinal caeca contain only comparably broad circular fibres. Within the female reproductive system, only circular fibres are present in the oviduct, vitelline duct and uterus. In contrast, the wall of the ootype displays closely arranged circular and longitudinal muscle fibres. Antisera to previously identified myoactive compounds (serotonin [5-hydroxytryptamine, 5-HT], neuropeptide F [Moniezia expansa] and GYIRFamide [Bdelloura candida, Dugesia tigrina]) were used as neuronal markers in a preliminary study of the spatial inter-relationships of specific nerve fibres and various muscle systems. Serotoninergic fibres innervate both suckers and also constitute a subtegumental nerve net. In males they provide innervation to the dorso-ventral muscle fibres of the gynaecophoric canal, and in females they innervate the circular and longitudinal muscle fibres of the ootype. Neuropeptide F and the FMRFamide-related peptide, GYIRFamide are both localized within nerve plexuses associated with the dorso-ventral fibres of the gynaecophoric canal, and are evident in the innervation of the ventral and oral sucker.
利用丝状肌动蛋白标记物异硫氰酸荧光素（FITC）偶联的鬼笔环肽，对成年雄性和雌性血吸虫的主要肌肉系统进行了研究。体壁肌肉组织包括一层环形纤维外层，其内部有一层紧密排列的短纺锤形纵向纤维以及内层对角纤维的网格状排列。在口吸盘和腹吸盘内可区分出3种纤维类型，即环形、放射状和纵向纤维。食管壁由环形和纵向纤维的网格状排列构成，而肠盲囊壁仅含有相当宽的环形纤维。在雌性生殖系统中，输卵管、卵黄管和子宫内仅存在环形纤维。相比之下，卵模壁显示出紧密排列的环形和纵向肌纤维。在一项关于特定神经纤维与各种肌肉系统空间相互关系的初步研究中，使用了针对先前鉴定的肌活性化合物（血清素[5-羟色胺，5-HT]、神经肽F[扩展莫尼茨绦虫]和GYIRF酰胺[念珠蛭、虎纹涡虫]）的抗血清作为神经元标记物。血清素能纤维支配两个吸盘，还构成皮下神经网。在雄性中，它们为抱雌沟的背腹肌纤维提供神经支配，在雌性中，它们支配卵模的环形和纵向肌纤维。神经肽F和FMRF酰胺相关肽GYIRF酰胺都定位于与抱雌沟背腹纤维相关的神经丛内，并且在腹吸盘和口吸盘的神经支配中很明显。
