Maule A G, Halton D W, Shaw C, Johnston C F
Comparative neuroendocrinology Research Group, Queen's University of Belfast, Northern Ireland, UK.
Parasitology. 1993 May;106 ( Pt 4):429-40. doi: 10.1017/s0031182000067184.
The central (CNS) and peripheral (PNS) nervous systems of the cyclophyllidean tapeworm, Moniezia expansa, were examined for the presence of cholinergic, serotoninergic and peptidergic elements using enzyme cytochemical and immunocytochemical techniques in conjunction with light and confocal scanning laser microscopy. Cholinesterase activity and 5-hydroxytryptamine- and regulatory peptide-immunoreactivities (IRs) were localized to the nerve fibres and cell bodies of all of the major neuronal components in the CNS of the worm, including the cerebral ganglia and connecting commissure, the 10 longitudinal nerve cords and associated transverse ring commissures. Although each of the 3 systems appeared well developed and comprised a significant portion of the nervous system, the serotoninergic constituent was the most highly developed, consisting of a vast array of nerve fibres and cell bodies distributed throughout the strobila of the worm. A close association of cholinesterase reactivity and peptide-IRs was evident throughout the CNS, indicating the possible co-localization of acetylcholine and neuropeptides. Within the PNS, cholinergic activity and serotoninergic- and peptidergic-IRs occurred in the subtegumental network of nerve fibres and somatic musculature. Although all 3 neurochemical elements were present in the acetabula, they were found in different nerve fibres; only cholinergic and peptidergic cell bodies were found. The common genital opening, vagina and ootype regions of the reproductive system displayed a rich innervation of all 3 types of neuronal populations. Within the peptidergic system, immunostaining with antisera raised to the C-terminus of the neuropeptide Y superfamily of peptides and the invertebrate peptides, neuropeptide F (M. expansa) and FMRFamide was the most prevalent. Limited positive-IR for substance P and neurokinin A were also recorded in the CNS of the worm.
利用酶细胞化学和免疫细胞化学技术，结合光学显微镜和共聚焦扫描激光显微镜，对扩展莫尼茨绦虫的中枢神经系统（CNS）和外周神经系统（PNS）进行检查，以确定其中胆碱能、5-羟色胺能和肽能成分的存在情况。胆碱酯酶活性以及5-羟色胺和调节肽免疫反应性（IRs）定位于该绦虫中枢神经系统中所有主要神经成分的神经纤维和细胞体，包括脑神经节和连接连合、10条纵神经索以及相关的横向环连合。尽管这三个系统中的每一个似乎都发育良好，并且在神经系统中占很大一部分，但5-羟色胺能成分发育最为完善，由分布在绦虫节片中的大量神经纤维和细胞体组成。在整个中枢神经系统中，胆碱酯酶反应性和肽免疫反应性之间存在明显的紧密关联，表明乙酰胆碱和神经肽可能共定位。在周围神经系统中，胆碱能活性以及5-羟色胺能和肽能免疫反应性出现在皮下神经纤维网络和体壁肌肉组织中。尽管所有三种神经化学元素都存在于吸盘，但它们存在于不同的神经纤维中；仅发现了胆碱能和肽能细胞体。生殖系统的共同生殖孔、阴道和卵模区域显示出所有三种神经元群体的丰富神经支配。在肽能系统中，用针对神经肽Y超家族肽的C末端以及无脊椎动物肽神经肽F（扩展莫尼茨绦虫）和FMRF酰胺产生的抗血清进行免疫染色最为普遍。在该绦虫的中枢神经系统中也记录到了对P物质和神经激肽A的有限阳性免疫反应。
