Manninen O, Kalendar R, Robinson J, Schulman A H
Institute of Biotechnology, University of Helsinki, Plant Genomics Laboratory, Viikki Biocenter, Finland.
Mol Gen Genet. 2000 Oct;264(3):325-34. doi: 10.1007/s004380000326.
Net blotch, which is caused by the fungus Pyrenophoral teres Drechs. f. teres Smedeg., presents a serious problem for barley production worldwide, and the identification and deployment of sources of resistance to it are key objectives for many breeders. Here, we report the identification of a major resistance gene, accounting for 65% of the response variation, in a cross between the resistant line C19819 and the susceptible cv. Rolfi. The resistance gene was mapped to chromosome 6H with the aid of two recently developed systems of retrotransposon-based molecular markers, REMAP and IRAP. A total of 239 BARE-1 and Sukkula retrotransposon markers were mapped in the cross, and the 30-cM segment containing the locus with significant resistance effect contained 26 of the markers. The type and local density of the markers should facilitate future map-based cloning of the resistance gene as well as manipulation of the resistance through backcross breeding.
网斑病由真菌柄锈菌（Pyrenophoral teres Drechs. f. teres Smedeg.）引起，对全球大麦生产构成严重问题，鉴定和利用其抗性资源是许多育种者的关键目标。在此，我们报告在抗性品系C19819与感病品种Rolfi的杂交后代中鉴定出一个主效抗性基因，其解释了65%的抗性变异。借助两个最近开发的基于反转录转座子的分子标记系统REMAP和IRAP，将该抗性基因定位到6H染色体上。在该杂交组合中共定位了239个BARE-1和Sukkula反转录转座子标记，包含具有显著抗性效应位点的30厘摩区段含有26个标记。这些标记的类型和局部密度将有助于未来基于图谱克隆抗性基因以及通过回交育种操控抗性。
