Cho C S, Kobayashi A, Takei R, Ishihara T, Maruyama A, Akaike T
School of Agricultural Biotechnology, Seoul National University, Suwon, South Korea.
Biomaterials. 2001 Jan;22(1):45-51. doi: 10.1016/s0142-9612(00)00143-5.
Cell modulators such as colchicine (CO), cytochalasin B (CY) and taxol (TX) loaded nanoparticles coated with carbohydrate-carrying polymers, as hepatocyte-specific targeting material using galactose ligands as recognition signals to asialoglycoprotein receptors were prepared by the diafiltration method. Effects of cell modulators from their loaded nanoparticles on morphology of hepatocytes were studied. Receptor-mediated endocytosis of the nanoparticles were examined by fluorescence and confocal laser microscopy. It was found that the shapes of most hepatocytes were changed for the CY-loaded, TX-loaded, or CO-loaded nanoparticles whereas their shapes were not changed in comparison with control when CY, TX, or CO were mixed with the nanoparticles. From the fluorescence and confocal laser microscopic studies, it is suggested that the nanoparticles coated with sugar-carrying polymers were internalized by the hepatocytes through the receptor-mediated mechanism.
采用渗滤法制备了负载秋水仙碱（CO）、细胞松弛素B（CY）和紫杉醇（TX）等细胞调节剂的纳米颗粒，这些纳米颗粒涂覆有携带碳水化合物的聚合物，以半乳糖配体作为识别信号作为去唾液酸糖蛋白受体的肝细胞特异性靶向材料。研究了负载细胞调节剂的纳米颗粒对肝细胞形态的影响。通过荧光和共聚焦激光显微镜检查纳米颗粒的受体介导内吞作用。结果发现，负载CY、TX或CO的纳米颗粒会使大多数肝细胞的形状发生改变，而当CY、TX或CO与纳米颗粒混合时，与对照组相比，肝细胞形状未发生改变。荧光和共聚焦激光显微镜研究表明，涂覆有含糖聚合物的纳米颗粒通过受体介导机制被肝细胞内化。
