Bates K M, Hansen L P, Green S P, Wallace D H, Green T J
Winona State University, Minnesota 55987, USA.
J Wildl Dis. 2000 Oct;36(4):774-8. doi: 10.7589/0090-3558-36.4.774.
Development of an in vitro culture system for infectious Dictyocaulus viviparus larvae made it possible to study the potential cross-transmission of D. viviparus between white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus). Between 26 September 1995-29 February 1996, six parasite-free bull calves were individually inoculated with 15 to 50 infective third stage larvae (L3)/kg of body weight cultured from adult D. viviparus collected from white-tailed deer. Three bull calves were simultaneously inoculated with 45 L3/kg of body weight recovered from cattle either by the Baermann technique or by in vitro culture as above. All three calves inoculated with the homologous cattle strain became patently infected while all six calves inoculated with the heterologous deer strain remained negative for the presence of D. viviparus in the feces and in the lungs upon necropsy.
用于感染性胎生网尾线虫幼虫的体外培养系统的开发，使得研究胎生网尾线虫在白尾鹿（弗吉尼亚鹿）和牛（黄牛）之间潜在的交叉传播成为可能。在1995年9月26日至1996年2月29日期间，六头无寄生虫的公牛犊分别接种了从白尾鹿采集的成年胎生网尾线虫培养的15至50条感染性第三期幼虫（L3）/千克体重。三头公牛犊同时接种了通过贝尔曼技术或上述体外培养从牛身上回收的45条L3/千克体重。接种同源牛株的所有三头犊牛都出现明显感染，而接种异源鹿株的所有六头犊牛在尸检时粪便和肺部均未检测到胎生网尾线虫。
