Desvoyes B, Scholthof K B
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77845-2132, USA.
FEBS Lett. 2000 Nov 17;485(1):25-8. doi: 10.1016/s0014-5793(00)02177-3.
The interactions between satellite panicum mosaic virus (SPMV) capsid protein (CP) and its 824 nucleotide (nt) single stranded RNA were investigated by gel mobility shift assay and Northwestern blot assay. SPMV CP has specificity for its RNA at high affinity, but little affinity for non-viral RNA. The SPMV CP also bound a 350 nt satellite RNA (satRNA) that, like SPMV, is dependent on panicum mosaic virus for its replication. SPMV CP has the novel property of encapsidating SPMV RNA and satRNA. Competition gel mobility shift assays performed with a non-viral RNA and unlabeled SPMV RNA and satRNA revealed that these RNA:protein interactions were in part sequence specific.
通过凝胶迁移率变动分析和蛋白质印迹法研究了卫星黍花叶病毒（SPMV）衣壳蛋白（CP）与其824个核苷酸（nt）的单链RNA之间的相互作用。SPMV CP对其RNA具有高亲和力的特异性，但对非病毒RNA的亲和力很低。SPMV CP还结合了一个350 nt的卫星RNA（satRNA），该卫星RNA与SPMV一样，其复制依赖于黍花叶病毒。SPMV CP具有包裹SPMV RNA和satRNA的新特性。用非病毒RNA以及未标记的SPMV RNA和satRNA进行的竞争凝胶迁移率变动分析表明，这些RNA与蛋白质的相互作用部分具有序列特异性。
