Higashitani A, Aoki H, Mori A, Sasagawa Y, Takanami T, Takahashi H
Institute of Genetic Ecology, Tohoku University, 2-1-1 Katahira, Sendai 980-8577, Japan.
FEBS Lett. 2000 Nov 17;485(1):35-9. doi: 10.1016/s0014-5793(00)02178-5.
A Chk2-like gene was identified in the genome of Caenorhabditis elegans. The putative gene product, termed Ce-chk-2 consists of 450 amino acid residues, and shows good homology with the Chk2/Cds1 gene family. The results of RNA-mediated interference (RNAi) indicated that the F1 generation from dsRNA injected animals grew to adulthood, but approximately 95% of their eggs (F2) died during early embryogenesis. Among the few surviving progeny, males (XO animals) arose at an abnormally high frequency (30%). In addition, 12 univalents were observed in full grown oocytes of the F1, while six bivalents were normally observed in wild-type oocytes. Ce-chk-2 gene expression increased in the adult stage, and their expression level decreased in the glp-4 mutant, which is defective in germ line proliferation. The radiation sensitivity of F1 embryos carrying Ce-chk-2 RNAi was not significantly affected.
在秀丽隐杆线虫的基因组中鉴定出一个类似Chk2的基因。推测的基因产物称为Ce-chk-2，由450个氨基酸残基组成，与Chk2/Cds1基因家族具有良好的同源性。RNA介导的干扰（RNAi）结果表明，注射dsRNA动物的F1代可发育至成年期，但它们约95%的卵（F2）在早期胚胎发育过程中死亡。在少数存活的后代中，雄性（XO动物）出现的频率异常高（30%）。此外，在F1的成熟卵母细胞中观察到12个单价体，而在野生型卵母细胞中通常观察到6个二价体。Ce-chk-2基因在成年期表达增加，在生殖系增殖有缺陷的glp-4突变体中其表达水平降低。携带Ce-chk-2 RNAi的F1胚胎的辐射敏感性没有受到显著影响。
