参与减数分裂重组的秀丽隐杆线虫recA样基因Ce-rdh-1的特性分析
Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination.
作者信息
Takanami T, Sato S, Ishihara T, Katsura I, Takahashi H, Higashitani A
机构信息
Institute of Genetic Ecology, Tohoku University, Sendai, Japan.
出版信息
DNA Res. 1998 Dec 31;5(6):373-7. doi: 10.1093/dnares/5.6.373.
A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.
在秀丽隐杆线虫基因组计划数据库中鉴定出一个类recA基因。该基因的推定产物称为Ce-rdh-1（秀丽隐杆线虫RAD51和DMC1/LIM15同源物1），由357个氨基酸残基组成。Ce-rdh-1的预测氨基酸序列与几种真核生物物种中RAD51型和DMC1/LIM15型基因的同一性为46%-60%。RNA干扰（RNA介导的干扰）结果表明，Ce-rdh-1的抑制会阻止F1代卵母细胞中六个二价体的染色体浓缩和交叉解离。卵子发生没有进入终变期。因此，所产生的所有卵（F2）在早期死亡。这些结果表明Ce-rdh-1参与减数分裂重组。
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