Kondo M, Kudo K, Kimura H, Inaba J, Kato K, Kojima S, Matsuyama T, Horibe K
Department of Pediatrics, Nagoya University School of Medicine, Japan.
Leuk Res. 2000 Nov;24(11):951-6. doi: 10.1016/s0145-2126(00)00071-0.
Quantification of AML1-MTG8 fusion transcripts was performed by using real-time reverse transcription-polymerase chain reaction (RT-PCR) and the clinical value of this method was evaluated in t(8;21)-positive acute myelogenous leukemia (AML). A t(8;21)-positive cell line, Kasumi-1, was used for constructing standard curves and the corrected AML1-MTG8 mRNA expression level relative to the expression of the GAPDH housekeeping gene was calculated. Bone marrow samples from 14 patients with t(8;21)-positive AML were sequentially examined. The corrected AML1-MTG8 expression level at diagnosis varied in the range from 0.4 to 2.7 (median, 1.5) among the patients. When samples at 1, 3 and 6 months were examined after diagnosis, the corrected AML1-MTG8 expression level was found to decrease sequentially in all but one. AML1-MTG8 fusion transcripts were also detected in four of eight samples from patients in remission for more than 1 year. In conclusion, real-time RT-PCR can provide a rapid and accurate quantification of AML1-MTG8 fusion transcripts. This system could be useful to reveal the prognostic relevance of minimal residual disease in t(8;21)-positive AML.
采用实时逆转录-聚合酶链反应（RT-PCR）对AML1-MTG8融合转录本进行定量分析，并评估该方法在t(8;21)阳性急性髓性白血病（AML）中的临床价值。使用t(8;21)阳性细胞系Kasumi-1构建标准曲线，并计算相对于管家基因GAPDH表达的校正AML1-MTG8 mRNA表达水平。对14例t(8;21)阳性AML患者的骨髓样本进行了连续检测。患者诊断时校正后的AML1-MTG8表达水平在0.4至2.7之间（中位数为1.5）。诊断后1、3和6个月检测样本时，发现除1例患者外，所有患者校正后的AML1-MTG8表达水平均依次下降。在缓解超过1年的患者的8份样本中，有4份也检测到了AML1-MTG8融合转录本。总之，实时RT-PCR可以快速、准确地定量AML1-MTG8融合转录本。该系统可能有助于揭示t(8;21)阳性AML中微小残留病的预后相关性。
