Degn P, Larsen K L, Duus J O, Petersen B O, Zimmermann W
Department of Civil Engineering, Aalborg University, Denmark.
Carbohydr Res. 2000 Oct 20;329(1):57-63. doi: 10.1016/s0008-6215(00)00176-2.
Glucose and maltose esters were synthesised in organic media by employing a lipase (E.C. 3.1.1.3) from Candida antarctica. In a second reaction step, a transglycosylation catalysed by a cyclodextrin glycosyltransferase (E.C. 2.4.1.19) from either Paenibacillus sp. F8 or Bacillus sp. strain no. 169 (DSM 2518) extended the degree of polymerisation (DP) of the carbohydrate moieties of the carbohydrate esters. The donor substrates used were either a cyclodextrin, a maltooligosaccharide or starch. The highest rate of low DP maltooligosaccharide ester formation was obtained when starch was used as glycosyl donor and caproyl maltose as glycosyl acceptor. The structures of two of the products were identified by 1H and 13C NMR and MALDI-TOF MS as capronate monoesters of maltotriose and maltotetraose, with the ester bond at C-6 of the second glucose unit from the reducing end.
利用来自南极假丝酵母的脂肪酶（E.C. 3.1.1.3）在有机介质中合成了葡萄糖酯和麦芽糖酯。在第二步反应中，由来自芽孢杆菌属F8或芽孢杆菌属菌株169（DSM 2518）的环糊精糖基转移酶（E.C. 2.4.1.19）催化的转糖基化反应提高了碳水化合物酯中碳水化合物部分的聚合度（DP）。使用的供体底物为环糊精、麦芽低聚糖或淀粉。当使用淀粉作为糖基供体和己酰麦芽糖作为糖基受体时，获得了最低聚合度麦芽低聚糖酯的最高形成速率。通过1H和13C NMR以及基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定了其中两种产物的结构，为麦芽三糖和麦芽四糖的己酸单酯，酯键位于还原端第二个葡萄糖单元的C-6位。
