Volc J, Sedmera P, Halada P, Prikrylová V, Haltrich D
Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague.
Carbohydr Res. 2000 Oct 20;329(1):219-25. doi: 10.1016/s0008-6215(00)00167-1.
Pyranose dehydrogenase purified to homogeneity from the mycelia of the basidiomycete fungus Agaricus bisporus catalyzed the oxidation of D-xylose at C-2 to D-threo-pentos-2-ulose (2-keto-D-xylose) and successively at C-3 to D-glycero-pentos-2,3-diulose (2,3-diketo-D-xylose) using 1,4-benzoquinone as an electron acceptor. The sites of oxidation were deduced from the spectroscopic analysis (MS, NMR) of the N,N-diphenylhydrazone derivatives of the reaction products.
