Jin H, Yang R, Li W, Lu H, Ryan A M, Ogasawara A K, Van Peborgh J, Paoni N F
Department of Cardiovascular Research, Genentech Incorporated, South San Francisco, California 94080, USA.
Am J Physiol Heart Circ Physiol. 2000 Dec;279(6):H2994-3002. doi: 10.1152/ajpheart.2000.279.6.H2994.
This study determined the effects of exercise training on cardiac function, gene expression, and apoptosis. Rats exposed to a regimen of treadmill exercise for 13 wk had a significant increase in cardiac index and stroke volume index and a concomitant decrease in systemic vascular resistance compared with both age-matched and body weight-matched sedentary controls in the conscious state at rest. In exercise-trained animals, there was no change in the expression of several marker genes known to be associated with pathological cardiac adaptation, including atrial natriuretic factor, beta-myosin heavy chain, alpha-skeletal and smooth muscle actins, and collagens I and III. Exercise training, however, produced a significant induction of alpha-myosin heavy chain, which was not observed in rats with myocardial infarction. No histological features of cardiac apoptosis were observed in the treadmill-trained rats. In contrast, apoptotic myocytes were detected in animals with myocardial infarction. In summary, exercise training improves cardiac function without evidence of cardiac apoptosis and produces a pattern of cardiac gene expression distinct from pathological cardiac adaptation.
本研究确定了运动训练对心脏功能、基因表达和细胞凋亡的影响。与年龄匹配和体重匹配的静息状态清醒久坐对照大鼠相比，接受13周跑步机运动训练的大鼠心脏指数和每搏输出量指数显著增加，同时全身血管阻力降低。在运动训练的动物中，已知与病理性心脏适应相关的几种标记基因的表达没有变化，包括心钠素、β-肌球蛋白重链、α-骨骼肌和平滑肌肌动蛋白以及I型和III型胶原蛋白。然而，运动训练显著诱导了α-肌球蛋白重链，这在心肌梗死大鼠中未观察到。在跑步机训练的大鼠中未观察到心脏细胞凋亡的组织学特征。相比之下，在心肌梗死动物中检测到凋亡心肌细胞。总之，运动训练可改善心脏功能，且无心脏细胞凋亡的证据，并产生一种与病理性心脏适应不同的心脏基因表达模式。
