Dodson M S
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, Arizona 85721-0088, USA.
Bioconjug Chem. 2000 Nov-Dec;11(6):876-9. doi: 10.1021/bc000049u.
Dimethyl suberimidate is a bifunctional reagent that is used for cross-linking the protein components of oligomeric macromolecules. In this report, dimethyl suberimidate is shown to specifically cross-link oligo(dT) of varying lengths to the DNA-binding subunits of a multimeric helicase-primase encoded by herpes simplex virus type 1. This result indicates that dimethyl suberimidate and other imidoester cross-linking reagents may be useful for characterizing the interaction of oligo(dT) with proteins that bind single-stranded DNA.
