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Lipid arrangement in fluid model membranes: analysis by cross-linking of phosphatidylethanolamines.

作者信息

Roth M R, Welti R

机构信息

Division of Biology, Kansas State University, Manhattan 66506.

出版信息

Biochim Biophys Acta. 1991 Apr 2;1063(2):242-6. doi: 10.1016/0005-2736(91)90377-k.

DOI:10.1016/0005-2736(91)90377-k
PMID:2012821
Abstract

Binary mixtures of fluid phase phosphatidylethanolamines at pH 10 were treated with the bifunctional cross-linking reagent dimethylsuberimidate. Analysis of the dimeric species formed demonstrated that the phospholipid species in dimyristoylphosphatidylethanolamine/dielaidoylphosphatidylethanolamine mixtures at 52 degrees C and dielaidoylphosphatidylethanolamine/dilauroylphosphatidylethanolamine mixtures at 41 degrees C were randomly arranged. Analysis of the dimeric species formed in dipalmitoylphosphatidylethanolamine/dioleoylphosphatidylethanolamine mixtures at 68 degrees C showed that this mixture was very close to being randomly arranged, with just a slight propensity of like phospholipid species to cluster.

摘要

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