阿尔茨海默病中核糖体RNA的定量评估。

Quantitative evaluation of the rRNA in Alzheimer's disease.

作者信息

da Silva A M, Payão S L, Borsatto B, Bertolucci P H, Smith M A

机构信息

Departamento de Morfologia, Disciplina de Genética, UNIFESP Escola Paulista de Medicina, Rua Botucatu, 740, Vila Clementino-CEP 04023-900, São Paulo, Brazil.

出版信息

Mech Ageing Dev. 2000 Dec 1;120(1-3):57-64. doi: 10.1016/s0047-6374(00)00180-9.

DOI:10.1016/s0047-6374(00)00180-9

PMID:11087904

Abstract

The ribosomal RNA (rRNA) genes are located in nucleolus during active transcription and are transcribed by RNA polymerase I. This group of genes is involved in transcription and translation processes which can modulate gene expression. The association between rRNA levels and aging has been reported. In the present study, we investigated the ratio of mature rRNA 28S and 18S in peripheral blood of 15 Alzheimer's disease (AD) patients, 15 elderly healthy controls and 15 healthy young controls. Our results showed a statistically significant decrease of the mature rRNA 28S/18S ratio in AD patients when compared with the elderly and young control groups. Thus we can suggest that there is a possible change in the transcriptional or maturation process or a preferential degradation of the 28S subunit in AD.

摘要

核糖体RNA（rRNA）基因在活跃转录期间位于核仁中，并由RNA聚合酶I转录。这组基因参与可调节基因表达的转录和翻译过程。rRNA水平与衰老之间的关联已有报道。在本研究中，我们调查了15名阿尔茨海默病（AD）患者、15名老年健康对照者和15名年轻健康对照者外周血中成熟rRNA 28S和18S的比例。我们的结果显示，与老年和年轻对照组相比，AD患者中成熟rRNA 28S/18S比例有统计学意义的下降。因此我们可以推测，在AD中，转录或成熟过程可能发生了变化，或者28S亚基存在优先降解。