Lehnert W, Wagner T O, Fürst M W, Künzer W
J Clin Chem Clin Biochem. 1979 Jun;17(6):363-7.
A new senitive and specific micromethod for determination of UDP-glucuronic acid is described Extracts from 2.5 mg of liver are incubated with twice washed guinea pig microsomes (as a source of UDP-glucuronyl transferase) and [14C]p-nitrophenol. The content of UDP-glucuronic acid is calculated from the [14C]p-nitrophenyl glucuronide/[14C]p-nitrophenol radioactivity ratio and the known amount of introduced [14C]p-nitrophenol. These values are corrected for losses of UDP-glucuronic acid by a calibration experiment, containing in addition to the aforementioned constituents, a known amount of UDP-glucuronic acid. The mean concentration of UDP-glucuronic acid in mice liver was found to be 171 mumol/kg wet weight.
本文描述了一种用于测定UDP-葡萄糖醛酸的灵敏且特异的微量方法。取2.5mg肝脏提取物，与经两次洗涤的豚鼠微粒体（作为UDP-葡萄糖醛酸基转移酶的来源）和[14C]对硝基苯酚一起孵育。根据[14C]对硝基苯基葡萄糖醛酸酯/[14C]对硝基苯酚的放射性比值以及引入的已知量的[14C]对硝基苯酚来计算UDP-葡萄糖醛酸的含量。通过校准实验对UDP-葡萄糖醛酸的损失进行校正，该校准实验除上述成分外，还含有已知量的UDP-葡萄糖醛酸。发现小鼠肝脏中UDP-葡萄糖醛酸的平均浓度为171μmol/kg湿重。
