一种用于测定皮摩尔量尿苷二磷酸葡萄糖醛酸的快速酶促方法。
A rapid enzymic procedure for the determination of picomole amounts of UDP-glucuronic acid.
作者信息
Singh J, Schwarz L R, Wiebel F J
出版信息
Biochem J. 1980 Aug 1;189(2):369-72. doi: 10.1042/bj1890369.
Abstract
A simple microassay for the determination of UDP-glucuronic acid was developed on the basis of the formation of benzo[a]pyrene 3-glucuronide catalysed by UDP-glucuronyltransferase of guinea-pig liver. As little as 1-5 pmol of UDP-glucuronic acid was detectable in extracts of heat-denatured probes of liver or cultured cells equivalent to 10-50 micrograms of cellular protein.
摘要
基于豚鼠肝脏UDP-葡萄糖醛酸基转移酶催化形成苯并[a]芘3-葡萄糖醛酸苷，开发了一种用于测定UDP-葡萄糖醛酸的简单微量测定法。在相当于10-50微克细胞蛋白的肝脏或培养细胞热变性探针提取物中，低至1-5皮摩尔的UDP-葡萄糖醛酸均可检测到。
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